Hibernation induces changes in the metacerebral neurons of Cornu aspersum: distribution and co-localization of cytoskeletal and calcium-binding proteins

Abstract

Pulmonate gastropods provide unique opportunities to examine physiological and biochemical adaptation strategies when cellular metabolic activity is reduced. In this study, cytochemical changes in metacerebral neurons of the cerebral ganglia were investigated in the garden snail Cornu aspersum during the hibernation phase. The immunocytochemical expression of three cytoskeletal markers: microtubule-associate protein 2-like (MAP-2-li), phosphorylated form of tau-like (P-Tau-li) and heavy subunit of neurofilaments-like (NF-H-li), and of two calcium-binding proteins: calmodulin-like (CaM-li) and parvalbumin-like (PV-li) was compared in active and hibernated snails. The immunopositivity for all the markers increased during hibernation versus activity in metacerebral neurons, with the notable exception of PV-li, which remained highly expressed during the whole annual cycle. Strongly positive aggregates of MAP-2-li and P-Tau-li were detected in the somata of hibernated snail neurons. P-Tau-li aggregates co-localized with CaM-li-labelled masses during hibernation. In addition, increased labelling of NF-H-li epitopes was associated with enhancement of CaM immunopositivity. These changes may reflect neural plasticity mechanisms mainly mediated by microtubule-associated proteins and CaM. Moreover, neuroprotective strategies may allow neurons to endure the prolonged hypometabolic conditions, taking into account that many of the functions controlled by the metacerebrum, such as feeding and movement, are suspended during hibernation. In this context, the molluscan ganglia model offers an easy opportunity to understand the molecular mechanisms behind these life cycle changes in cell physiology and to investigate possible cytological similarities among distantly related animals that adapt to the same environmental challenges through hibernation.