Abstract
ObjectiveThe primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC).MethodsFirstly, HHIP gene expression data of NSCLC tissues and normal tissues were obtained from GSE18842/GSE19804/GSE43458 databases of the Gene Expression Omnibus (GEO) database and then validated by TCGA NSCLC database in a cohort of 1027 cases of NSCLC patients and 108 cases of normal people. A chi-square test was used to analyze the relationship between HHIP expression and clinicopathological characteristics of NSCLC. The expression levels of HHIP in NSCLC cells were detected by quantitative-real time PCR. The function of HHIP was investigated by a series of in vitro assays. CCK-8, wounding healing, Transwell invasion assay were utilized to explore the mechanisms of HHIP.ResultsHHIP mRNA were significantly down-regulated in NSCLC in three GEO databases and TCGA database (P<0.05). This result was confirmed in NSCLC cell lines by qRT-PCR analysis, its expression in normal NSCLC cell line BEAS-2B was significantly higher than that in NSCLC cells. Chi-square test results showed that the low expression of HHIP was correlated with gender, cancer type, TNM stage and tumor size. Functional experimental results showed that over-expressing HHIP significantly decreased the ability of cell proliferation, migration and invasion in NSCLC cells (P<0.05).ConclusionOverall, the above results indicated that HHIP could regulate proliferation, migration and invasion, and could be used as a judging criterion for identifying NSCLC classification and stage.
Highlights
Lung cancer is one of the most common malignant tumors in recent years[1]
HHIP mRNA were significantly down-regulated in non-small cell lung cancer (NSCLC) in three Gene Expression Omnibus (GEO) databases and TCGA database (P
Functional experimental results showed that over-expressing HHIP significantly decreased the ability of cell proliferation, migration and invasion in NSCLC cells (P
Summary
Lung cancer is one of the most common malignant tumors in recent years[1]. According to histology and pathology, it can be divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). NSCLC is a common type of lung cancer and accounts for about 85% of the total amount. The pathological type of NSCLC includes lung adenocarcinoma, lung squamous cell carcinoma and large cell lung cancer[2]. The latest statistics shows that about 26% tumor patients died from lung cancer[3]. The mortality of lung cancer is still high in our country and its therapeutic efficacy is limited. The discussion of the mechanism of the occurrence and development of NSCLC is of great importance, which may provide clinical therapy with new strategies
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