Abstract
Two strains of Saccharomyces cerevisiae and one of Candida parapsilosis were grown on biotin-deficient and on biotin-rich media and the hexokinase activity in cell-free extracts determined. Extracts from C. parapsilosis grown on the biotin-deficient medium had more hexokinase activity than extracts from the organism grown on biotin-rich medium. The hexokinase activity of extracts from these yeasts grown on biotin-deficient medium was not increased by biotin, or inhibited by avidin dissolved in saline. The results do not confirm the possibility of a direct role for biotin in hexokinase activity.
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