Heterotrophic nitrification-aerobic denitrification characteristics and antibiotic resistance of two bacterial consortia from Marinomonas and Halomonas with effective nitrogen removal in mariculture wastewater

Abstract

Heterotrophic nitrification-aerobic denitrification (HNAD) characteristics and antibiotic resistance of two bacterial consortia, Marinomonas communis &Halomonas titanicae (MCH) and Marinomonas aquimarina &Halomonas titanicae (MAH), and their single isolates (MC, MA, and H) were determinated in this study. When cultured in sole and mixed N-source media (NH4+-N and/or NO2−-N of 10 mg/L), MCH and MAH exhibited greater efficiency and stability of inorganic-N removal than single isolates, and these strains preferred to remove NH4+-N by simultaneous HNAD in mixed N-source media. Meanwhile, 45%–70% of NH4+-N and/or NO2−-N was mainly converted to organic nitrogen (15%–25%) and gaseous nitrogen (30%–40%) by these strains, and more inorganic-N was transformed to intracellular-N by MCH and MAH via assimilation instead of gaseous-N production by denitrification. Both isolates and their consortia had the maximal NH4+-N or NO2−-N removal efficiency above 95% under the optimum conditions including temperature of 20–30 °C, C/N ratios of 15–20, and sucrose as carbon source. Interestingly, bacterial consortia performed greater nitrogen removal than single isolates under the low temperature of 10 °C or C/N ratios of 2–5. In real mariculture wastewater, MCH and MAH also showed higher NH4+-N removal efficiency (65%–68%) and more stable cell quantity (4.2–5.2 × 108 CFU/mL) than single strains, due to the interspecific coexistence detected by bacterial quantitation with indirect immunoassay. Additionally, these isolates and consortia had stronger resistances to polypeptides, tetracyclines, sulfonamides, furanes, and macrolides than other antibiotics. These findings will be conducive to the applications of HNAD bacteria of Marinomonas and Halomonas on reducing nitrogen pollution in mariculture or other saline environments.