Heterotrophic microbial activities and nutritional status of microbial communities in tropical marsh sediments of different salinities: the effects of phosphorus addition and plant species

Abstract

Oligotrophic, phosphorus (P) limited herbaceous wetlands of northern Belize are being impacted by P loading from fertilizer runoff. P enrichment causes a shift in autotroph communities from a microphyte (cyanobacterial mats, CBM) to macrophyte (Eleocharis spp., Typha domingensis) dominated system. To document potential effects of P, salinity, and macrophyte species on the heterotrophic microbial community nutritional status (represented especially by specific phospholipids fatty acids and specific respiration rate), biomass and activities, we took soil samples from established P enrichment plots in replicated marshes of two salinity levels. P addition increased microbial biomass carbon (C), nitrogen (N) and P, as well as soil nutrient transformation rates (nitrogenase activity, N mineralization and immobilization, methanogenesis). The effect of plant species (Eleocharis vs Typha sites) was generally lower than the effect of P addition (CBM vs Eleocharis sites) and was most evident at the low salinity sites, where Eleocharis dominated plots had enhanced nitrogenase activity and P microbial immobilization. Salinity reduced the overall rates of microbial processes; it also weakened the positive effect of both P addition and plant species on microbial activities. Lastly, the amount of N stored in microbial cells, likely in form of osmoprotective compounds, was enhanced by salinity.