Heterotrophic denitrification at extremely high salt and pH by haloalkaliphilic Gammaproteobacteria from hypersaline soda lakes

Abstract

In this paper we describe denitrification at extremely high salt and pH in sediments from hypersaline alkaline soda lakes and soda soils. Experiments with sediment slurries demonstrated the presence of acetate-utilizing denitrifying populations active at in situ conditions. Anaerobic enrichment cultures at pH 10 and 4 M total Na+ with acetate as electron donor and nitrate, nitrite and N2O as electron acceptors resulted in the dominance of Gammaproteobacteria belonging to the genus Halomonas. Both mixed and pure culture studies identified nitrite and N2O reduction as rate-limiting steps in the denitrification process at extremely haloalkaline conditions.Electronic supplementary materialThe online version of this article (doi:10.1007/s00792-008-0166-6) contains supplementary material, which is available to authorized users.