Heterotrophic bacterioplankton production and grazing mortality rates in an Ethiopian rift‐valley lake (Awassa)

Abstract

SUMMARY. 1. Heterotrophic bacterioplankton growth and production rates were estimated in a tropical lake by various methods. Mean growth rates, determined by tritiated thymidine incoporation into DNA, frequency of dividing cells and increase in cell density varied between 0.013 and 0.014 (with a range of 0.006–0.026) h−1 corresponding to bacterial production of 1.16–1.22 (0.34–3.63) mg C m−3 h−1.2. Heterotrophic bacterial production estimated from oxygen and inorganic carbon consumption in the dark were compared with these values. The oxygen method gave similar results, while values from dark carbon uptake were as much as 2.5 times higher.3. Although the different estimates of rates of bacterial production showed different patterns, the existence of spatial (vertical) and temporal (diel and seasonal) variation was demonstrated. Bacterial production was 13–41% of the net primary production and 10–30% of gross primary production.4. Bacterial grazing mortality rate was estimated from size‐fractionation and metablic inhibitor experiments. Average grazing rates were between 0.34 and 3.77 mg C m−3 h−1 corresponding to 76–120% of the mean bacterial production rate. Organisms 1–12 μm in size, possibly mainly ciliates. were implicated as important bacterial grazers.