Heteropaternity of twins in ring-tailed lemurs (Lemur catta).

Abstract

This project investigated paternity among 14 sets of twins born into a colony of ring-tailed lemurs (Lemur catta) on St. Catherines Island, GA. Female L. catta commonly mate with >1 male, and twins of different sexes confirm that dizygotic twinning can occur in this species. However, no study has previously evaluated twins using genetic data to measure the proportion of mono versus dizygotic twinning in L. catta, and no study has tested for heteropaternity (sirehood by two different males) in this species. We predicted that L. catta would show a predominance of dizygosity and evidence for heteropaternity. Paternity was determined for 28 infants (14 sets of twins) via paternity exclusion analyses using 10 polymorphic microsatellite markers, and data were collected on sexual behavior across four mating seasons during the first cycle of mating in each year, which overlapped with the conceptive periods of these infants. All twins were found to be dizygotic, as evidenced by dissimilar multilocus genotypes between the two infants of each twin pair. Heteropaternity was found in 3 of 14 (21% of) twin pairs, whereas the remaining 11 pairs of twins (79%) shared the same sire. In one case of heteropaternity, one sire was a resident male, and one sire was an extra-group male. An extra-group male also sired one of the same-sire twin pairs. All other sires of twins were group residents. Female multiple mating, which is common to many primates including L. catta, can be viewed as an adaptive reproductive strategy whose function-in addition to fertility assurance or stimulating sperm competition-may be to increase the genetic diversity of a female's offspring, even among infants born in the same birth event. Therefore, during the course of primate evolution, the adaptive benefits of heteropaternity would be expected to have contributed to positive selection for female promiscuity.