Abstract
The non-ionic biosurfactant serrawettin W1 is a lipopeptide produced by red-pigmented strains of Serratia marcescens which shows antimicrobial, antitumor and plant protecting properties. Here, we report a simple method for heterologous production of this biosurfactant. S. marcescens strain DSM12481 was identified as a novel serrawettin W1 producer and the respective nonribosomal peptide synthetase gene swrW was cloned and expressed in Escherichia coli BL21 Gold. Chemical analysis of heterologous serrawettin W1 revealed that E. coli mainly produced serrawettin with C10 fatty acids as does S. marcescens. Additionally, serrawettin species with longer fatty acids (C13, C14) were detected in S. marcescens which were absent in E. coli. The expression system described here paves the way for the large scale production of this biotechnologically important biosurfactant.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
