Abstract
Background Extracellular hydrolytic enzymes such as α-amylases are widely used in diverse applications in different industrial areas. α-amylase (EC 3.2.1.1) is an important endo-type carbohydrase that hydrolyzes α-1,4 glycosidic linkages of D-glucose oligomers and polymers. This enzyme has been found in organisms of the three Domains, being a key enzyme of carbohydrate metabolism. Haloferax mediterranei is an extremely halophilic Archaea that requires high salt concentrations to grow. This microorganism is able to grow in a minimal medium with ammonium acetate as the only source of carbon and nitrogen.H. mediterranei shows α-amylase extracellular activity when grows in this minimal medium in the presence of starch. The main role of this enzyme is the starch metabolism in the extracellular medium, so a lot of microorganisms depend on amylases for survival [1].
Highlights
Extracellular hydrolytic enzymes such as α-amylases are widely used in diverse applications in different industrial areas. α-amylase (EC 3.2.1.1) is an important endo-type carbohydrase that hydrolyzes α-1,4 glycosidic linkages of D-glucose oligomers and polymers
The extracellular halophilic H. mediterranei α-amylase has been purified to electrophoretic homogeneity [2]
The recombinant protein was mainly obtained as inclusion bodies, a small amount of protein was presented in the cytoplasmic soluble fraction (Figure 1)
Summary
Heterologous overexpression of a halophilic α-amylase Vanesa Bautista*, Julia Esclapez, Rosa Ma Martínez-Espinosa, Francisco Pérez-Pomares, Mónica Camacho and Ma José Bonete. Address: Department of Biochemistry and Molecular Biology, University of Alicante, 03080 Alicante, Spain * Corresponding author. Open Access from The 4th Recombinant Protein Production Meeting: a comparative view on host physiology Barcelona, Spain. 21–23 September 2006 Published: 10 October 2006 Microbial Cell Factories 2006, 5(Suppl 1):P13 doi:10.1186/1475-2859-5-S1-P13. The 4th Recombinant Protein Production Meeting: a comparative view on host physiology The organisers would like to thank Novozymes Delta Ltd who generously supported the meeting. Meeting abstracts – A single PDF containing all abstracts in this supplement is available here http://www.biomedcentral.com/content/pdf/1475-2859-5-S1-info.pdf
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