Abstract
Several Saccharomyces cerevisiae strains with a super-secreting phenotype have been transformed using a secretion plasmid containing the LAC4 gene and have proven to be effective in the secretion of Kluyveromyceslactis β-galactosidase. The strain CGY1585 (ssc1-1) showed the highest secretion (1.7 EU ml−1) in the culture medium. As far as we know, Kluyveromyceslactis β-galactosidase is the largest sized protein and the only intracellular one among those secreted by these mutants hitherto. The recombinant strains all grew in lactose media.
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