Abstract
Covering: 2013 to June 2018 Heterologous expression of natural product biosynthetic pathways is of increasing interest in microbial biotechnology, drug discovery and optimization. It empowers not only the robust production of valuable biomolecules in more amenable heterologous hosts but also permits the generation of novel analogs through biosynthetic engineering. This strategy also facilitates the discovery of novel bioactive compounds following the functional expression of cryptic biosynthetic gene clusters (BGCs) from fastidious original producers or metagenomic DNA in surrogate hosts, thus facilitating genome mining in the post-genomic era. This review discusses recent advances and trends pertaining to the heterologous production of bacterial natural products, with an emphasis on new techniques, heterologous hosts, and novel chemistry since 2013.
Highlights
Technical advances in heterologous expression of biosynthetic gene clusters (BGCs)2.2 Considerable factors affecting heterologous expression number of naturally occurring metabolites exhibiting structural
We focus on bacterial natural products, among which polyketides and peptides are the two most abundant types
non-ribosomal peptide synthetases (NRPSs), RiPP biosynthesis is initiated by a ribosomally This review aims to provide an inspiring insight into generated precursor peptide encoded by a structure-encoding strategies for heterologous expression of bacterial biosyn
Summary
2.2 Considerable factors affecting heterologous expression number of naturally occurring metabolites exhibiting structural. 4. Yield improvement by heterologous expression thereof.[2,3] Associated products have been used as antibiotics, 30 5. Identi cation of biosynthetic pathways for known natural antitumor agents, immunosuppressive agents, insecticides, 30 products by heterologous expression and herbicides as well as antiparasitic agents.[4,5] Microorgan-. 6. Discovery of novel compounds by heterologous expression isms are considered to be one of the most proli c sources of of BGCs from cultured bacteria novel natural products for two reasons: (1) historically, micro-. 7. Discovery of novel compounds by heterologous expression a very small fraction of microorganisms have been exploited for of DNA from uncultured bacteria and metagenomic DNA the production of bioactive secondary metabolites; there
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