Abstract
Biphenyl 2,3-dioxygenase (BphA) is an enzyme that participates in initiation step of the pathway of biphenyl degradation in bacteria. Biphenyl is an aromatic hydrocarbon including two benzene rings that causes a serious environmental pollution. This report presents the preliminary results on the cloning and expression of BphA gene of B. cepacia strains DF2 and DF4 which were isolated from dioxin contaminated soil in Vietnam. The coding DNA sequences of BphA genes are 897 bp long and 99% identical. Recombinant BphA enzyme (33 kDa) has strongly expressed in E. coli M15 after induction of 0.5 mM IPTG for 4 h at 37ºC.
Highlights
Biphenyl, one of four substrates of biphenyl 2,3-dioxygenase, is an aromatic hydrocarbon in which two benzene rings are connected to each other
This report presents the preliminary results on the cloning and expression of biphenyl dioxygenase subunit alpha (BphA) gene of B. cepacia strains DF2 and DF4 which were isolated from dioxin contaminated soil in Vietnam
Biphenyl 2,3-dioxygenase, called biphenyl dioxygenase subunit alpha (BphA), is an enzyme that belongs to the family of oxidoreductase participating in initiatory step of the pathway of biphenyl degradation in bacteria (Leewis et al, 2016)
Summary
One of four substrates of biphenyl 2,3-dioxygenase, is an aromatic hydrocarbon in which two benzene rings are connected to each other. This report presents the preliminary results on the cloning and expression of BphA gene of B. cepacia strains DF2 and DF4 which were isolated from dioxin contaminated soil in Vietnam. Recombinant BphA enzyme (33 kDa) has strongly expressed in E. coli M15 after induction of 0.5 mM IPTG for 4 h at 37oC.
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