Abstract
The rotational correlation time of the lipid probe 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC) is measured using fluorescence anisotropy for two lipid species. We measure the rotational diffusion in a monolayer of 1,2-Didecanoyl-sn-glycero-3-phosphocholine (DPPC) which displays a phase transition at room temperature from the liquid-expanded to the liquid-condensed phase. The constant rotational diffusion of the probe throughout the phase transition reflects the measurement of dynamics in only the liquid-expanded phase. We contrast the dynamic changes during this phase coexistence to the continuous density increase observed in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at room temperature. We observe a non-exponential decay of the probe diffusion consistent with heterogeneity of the orientational dynamics.
Highlights
The rotational correlation time of the lipid probe 1-palmitoyl-2-{6-[(7-nitro-2-1,3benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC) is measured using fluorescence anisotropy for two lipid species
We measure the rotational diffusion in a monolayer of 1,2-Didecanoyl-sn-glycero-3-phosphocholine (DPPC) which displays a phase transition at room temperature from the liquidexpanded to the liquid-condensed phase
We measure the distribution of rotational correlation times for lipids at the air-water interface to characterize the degree of dynamic heterogeneity in single constituent lipid monolayers
Summary
In this study, we consider a model lipid system that can provide a baseline for describing the distribution of dynamic clustering in free-standing lipid structures To this end, we measure the distribution of rotational correlation times for lipids at the air-water interface to characterize the degree of dynamic heterogeneity in single constituent lipid monolayers. We measure the distribution of rotational correlation times for lipids at the air-water interface to characterize the degree of dynamic heterogeneity in single constituent lipid monolayers Such observations have implications for the heterogeneity for more complex lipid structures and biological membranes. A monolayer of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) near room temperature displays a continuous isotherm, indicating a single liquid phase (Figure 1(b)), and no observable partitioning of the probe in fluorescence microscopy images. This work offers insight into the local organization of lipid structures which has important consequences protein selfassembly in biological membranes
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