Abstract
Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) is a downstream target gene of the Wnt/β-catenin signaling pathway and identified as a marker of cancer stem-like cells of colorectal carcinoma (CRC). Here, the heterogeneous expression pattern of Lgr5 and its clinical significance were studied by the method of immunohistochemistry in 204 CRC tumors at various pTNM stages. Lgr5 expression was found in 82.4% (168/204) cases, significantly more common in neoplastic cells at the infiltrative front (n = 59.5%, 110/185) or at the expanding front (n = 36.4%, 59/162) than at the tumor center (n = 16.7%, 34/204; P < 0.01). Tumor budding (TB) was discovered with significantly higher Lgr5 expression (n = 39.3%, 57/145, P = 0.03) and significantly positively correlated between Lgr5 expression and TB grade (r = 0.19, P = 0.02). Additionally, both positive Lgr5 expression and a high TB grade were significantly correlated to the depth of tumor invasion, lymph node metastasis, pTNM stage, and perineural invasion (P < 0.01). The study results suggest that heterogeneous expression of Lgr5 may be a risk factor for local invasion and distant metastasis of CRC.
Highlights
3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Immunostaining of Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) was performed in 204 cases of CRC, and the results showed that Lgr5 expression in CRC tumors staged as pTNM I-IV was heterogeneous, but significantly higher at the infiltrating front than at the expanding front, or at the tumor epicenter
High Lgr5 expression in tumor buds was significantly correlated to high tumor budding, positively correlated to the depth of invasion, lymph node metastasis, pTNM stage, and perineural invasion
Summary
3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The study results suggest that heterogeneous expression of Lgr may be a risk factor for local invasion and distant metastasis of CRC. Neoplastic cells of CRC was significantly more often found at the invasive front than at the tumor center; high Lgr expression was detected in the region of tumor budding (TB), residual cancer tissues surrounding the necrotic foci, and distant metastatic organs of 42 CRC cases staged as pTNM IV. These results indicated that Lgr over-expression was possibly related to proliferation, invasion, and prognosis of CRC [6]. Lgr at the tumour center, invasive front, and TB of CRC staged as pTNM I–IV and explore its relationship with clinicopathological and prognostic characteristics of CRC
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