Abstract
Brassica napus (B. napus) is the world's most widely grown temperate oilseed crop. Although breeding for human consumption has led to removal of erucic acid from refined canola oils, there is renewed interest in the industrial uses of erucic acid derived from B. napus, and there is a rich germplasm available for use. Here, low- and high-erucic acid accessions of B. napus seeds were examined for the distribution of erucic acid-containing lipids and the gene transcripts encoding the enzymes involved in pathways for its incorporation into triacylglycerols (TAGs) across the major tissues of the seeds. In general, the results indicate that a heterogeneous distribution of erucic acid across B. napus seed tissues was contributed by two isoforms (out of six) of FATTY ACYL COA ELONGASE (FAE1) and a combination of phospholipid:diacylglycerol acyltransferase (PDAT)- and diacylglycerol acyltransferase (DGAT)-mediated incorporation of erucic acid into TAGs in cotyledonary tissues. An absence of the expression of these two FAE1 isoforms accounted for the absence of erucic acid in the TAGs of the low-erucic accession.
Highlights
Brassica napus (AACC, 2n = 38) is an allotetraploid oilseed plant species formed by the hybridization of two diploid species of Brassica rapa (AA, 2n = 20) and Brassica oleracea (CC, 2n = 18) about 7,500 years ago (Chalhoub et al, 2014; An et al, 2019)
Comparative Analysis of Two B. napus Seeds Differing in Erucic Acid Content
Two natural B. napus accessions that differ in their erucic acid content were selected, WH3401, which contains ca. 34% erucic acid, and WY20 with almost no erucic acid content
Summary
Brassica napus (AACC, 2n = 38) is an allotetraploid oilseed plant species formed by the hybridization of two diploid species of Brassica rapa (AA, 2n = 20) and Brassica oleracea (CC, 2n = 18) about 7,500 years ago (Chalhoub et al, 2014; An et al, 2019). It is the third largest oil crop in the world and accounts for approximately 15% of the vegetable oil used for human consumption (Wells et al, 2014; Liu et al, 2016; Carruthers et al, 2017; Kaur et al, 2019). Low erucic acid content (< 2%) is a major evaluation index for edible rapeseed cultivars (Hristov et al, 2011; Yan et al, 2015), and reducing erucic acid content has been a major goal for rapeseed
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