Heterogeneous chemiluminescent assay (generalizing paper)

Abstract

The results of applying heterogeneous chemiluminescent analysis for determination of micro-components are generalized, and the possibility of using this method for determination of toxic anions in waters is demonstrated. Anions undergo conversion beforehand, which causes formation of new analytical forms, i.e., derivates being active in chemiluminescent reactions with luminol. If volatile products are generated as analytical forms, they undergo gas extraction from the matrix with detection using a chemiluminescent indicator in the carrier gas stream at the interface gas-liquid (aqueous solution of luminol). Thus, the techniques for determination of Cl−, Br−, I−, ClO 3 − , BrO 3 − , IO 3 − , NO 3 − , NO 2 − , S2, CrO 4 2− , AsO 2 − , Cl2 and chloramines are developed. The detection limit (DL) is 0.5–5.0 μg/l, and the analysis time is 2–9 min. In another case, products of conversion are sorbed from the solution on a solid sorbent, and detection is carried out by a luminol solution on the solid surface. A series of heteropoly acids (phosphorus, arsenic, silicon, and germanium) and their ion associates with cation surfactants, along with other large anions, are determined in the form of sorbable ion associates. The DL is 0.02–0.7 μg/l, and the analysis time is 30 min. The advantages of application of heterogeneous chemiluminescent analysis over analysis in homogeneous media are indicated, and the prospects for development of the theoretical bases and field of application are demonstrated.