Abstract
Rabbits were chronically bled, with Fe replacement, every 3–4 days for 21 days. Their platelet count and megathrombocyte number (large-heavy, young platelets) increased 1.7-and 2.3-fold, respectively. Eight of the 11 enzymes of the Embden-Meyerhof pathway increased 2–5-fold in activity per g wet weight during the period of blood letting. The five related enzymes (phosphoglucomutase, glucose 6- P dehydrogenase, 6- P gluconic dehydrogenase, glutathione reductase, and α-glycerol- P dehydrogenase) as well as the three Embden-Meyerhof pathway enzymes (aldolase, enolase, and pyruvate kinase) did not increase in activity over basal values. It is concluded that chronic blood loss with Fe replacement leads to a specific increase in enzyme activity of 8 of the 11 Embden-Meyerhof pathway enzymes.
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