Heterogeneity of mean sarcomere length in different fibres: effects on length range of active force production in rat muscle.

Abstract

Isometric active length-force characteristics of rat semimembranosus lateralis muscles [rats, n = 6, body mass = 292 (SD 9) g] were recorded. Muscles were photographed during the force plateaus of the tetani to determine lengths of proximal, intermediate and distal fibres. From the mean number of sarcomeres in series in those fibres, mean sarcomere length at different muscle lengths was calculated. The heterogeneity of mean sarcomere lengths for each muscle was quantified according to the coefficient of variation of sarcomere lengths at muscle optimal length. Absolute muscle length changes were equal to fibre length changes. At all muscle lengths, mean sarcomere lengths in the distal fibres were significantly greater than those in proximal and intermediate fibres. The heterogeneity of mean sarcomere lengths augmented the length range of active force production between muscle optimal length and active slack length by about 38% to 43%. We concluded that in a muscle with a low degree of pennation, the heterogeneity of mean sarcomere lengths should be considered as a substantial contributor to the length range over which active force can be produced. In our experiment, the length ranges of active force production between optimal and slack length showed considerable differences (range 18.7-24.9 mm). The coefficient of correlation between length ranges and mean number of sarcomeres in series in various muscle regions was extremely low (r = -0.14) and not significant. The coefficient of correlation between length ranges and heterogeneity was high (r = 0.88) and significant. These data would suggest that muscles with a similar number of sarcomeres in series can exhibit quite different functional characteristics.