Abstract
Pestivirus are responsible for cosmopolitan diseases affecting cattle, pigs and other ruminants, presenting a wide range of clinical manifestations, with relevant impact on zootechnic production. Understanding genomic characteristic and virus taxonomy is fundamental in order to sustain control and prophylactic programs. Given the recent various studies reporting a relatively high number of new strains, in particular from Asian countries, in the present study, six hundred-fifty-one genomic sequences have been considered applying the palindromic nucleotide substitutions method for genotyping. Based on the secondary structure analysis of the 5’ untranslated region of RNA, sequence characteristics among Asian genomic clusters within the different Pestivirus species suggested geographic segregation and occurrence of micro-evolutive steps in the genus evolutionary history. This aspect was particularly evident in atypical sequences originated from China or Turkey, indicating risk of diffusion by animals and products trade or contamination of biological products as bovine calf serum, with potential diagnostic and control difficulties.
Highlights
Species of the genus Pestivirus of the family Flaviviridae [1] are responsible for cosmopolitan disease affecting cattle pig and other ruminants, presenting a wide range of clinical manifestations, with relevant impact on zootechnic production
Asian atypical sequences have been reported in other Pestivirus species as BVDV-3 (Pestivirus H) and Classical swine fever virus (CSFV) (Pestivirus C) [11] [12] [13]
Taking into account that a certain confusion in the nomenclature of the increasing number of Pestivirus genetic variants was evident and it is still a matter of concern, indicating that the need for harmonization [14] [15] [16], and the utility to have an alternative evaluation method are always interesting in any discipline, the palindromic nucleotide substitutions (PNS) method [17] [18] was proposed for an analytical procedure not limited to primary structure analysis but extended to secondary structure, focusing on strategic genomic sequences corresponding to the internal ribosome entry site (IRES), in the 5’ untranslated region (UTR), responsible for translational, transcriptional and replication events in pestiviruses [19]
Summary
Species of the genus Pestivirus of the family Flaviviridae [1] are responsible for cosmopolitan disease affecting cattle pig and other ruminants, presenting a wide range of clinical manifestations, with relevant impact on zootechnic production. Asian atypical sequences have been reported in other Pestivirus species as BVDV-3 (Pestivirus H) and Classical swine fever virus (CSFV) (Pestivirus C) [11] [12] [13]. In order to evaluate more exhaustively the taxonomical segregation of the genus Pestivirus in Asian countries, the 5’-UTR genomic sequences of the BVDV-1, BVDV-2 (Pestivirus B), BVDV-3, Border Disease virus (BDV) (Pestivirus D) and CSFV species were analyzed and compared applying the PNS genotyping method, encompassing all Pestivirus species circulating in domestic animals in Asia and excluding the pestiviruses from chiropters
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
