Abstract

Restriction fragment length polymorphisms (RFLP) of polymerase chain reaction (PCR) amplified nuclear ribosomal DNA intergenic spacer (IGS) region were used to analyze genetic variability among Pyrenophora graminea isolates collected from diverse regions of Syria. The IGS region was variable among isolates and 20 unique haplotypes were detected. The phylogenetic analysis of IGS-RFLP profiles enabled the isolates to be clustered into three different groups. There was limited resolution between clustering isolates and their origins. The phylogeographic distribution pattern suggests a seed-borne dispersal of Pyrenophora graminea.

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