Heteroflocculation of Amidine Polystyrene Latex and Anticarsia gemmatalis Nucleopolyhedrovirus as a Model System for Studying Sunlight Protection

Abstract

Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) is a baculovirus specific for the control of an important soybean defoliator. The baculovirus is comprised of double-stranded DNA, occluded in a proteinaceous structure called a polyhedron. Ultraviolet sunlight is the most destructive factor that affects the persistence of the virus in the field. In the present study, we use a model system wherein the pathogen is covered by another particle of opposite charge in order to test the effectiveness of a physical barrier as a protection against sunlight. Heteroflocculation experiments were carried out using two different age batches of AgMNPV and amidine polystyrene latex particles. The assessment of heteroflocculation was achieved by zeta potential and adsorption isotherm measurements, and by scanning electron microscopy. Despite the great difference in potentials between latex particles and the baculovirus, low-affinity isotherms were obtained in both pure water and 0.1 mM KCl. Adsorbed latex particles were easily washed out from the polyhedron surface. This low affinity could be attributed to the presence of a strongly repulsive hydration force of short range operating on the system. The results suggest that the failure to obtain a good physical barrier against sunlight might be attributed to the difficulty in keeping the polyhedron surface covered.