Heterochromatic stray light in UV absorption spectrometry: a new test method

Abstract

A new method of estimating the amount of heterochromatic stray light in UV spectrophotometers is described. The method uses the same solution filters with sharp UV absorption edges as ASTM Test Method E387, but one measures the apparent absorbance of a 10-mm path-length cell in the sample beam relative to a 5-mm cell in the reference beam. Scanning toward shorter wavelengths, one records an apparent absorbance maximum which is a direct measure of the stray light. This method was found to be in satisfactory agreement with the ASTM method in comparative tests of several spectrophotometers at different wavelengths between 200 and 390 nm, using KC1, KI, Nal, acetone, and NaNO(2) solution filters. The new method proved to be simpler, the main advantage being that the apparent absorbance maximum occurs at considerably lower scale values than the corresponding absorbance plateau measured by the ASTM method. This reduces the need for successive attenuations of the reference beam every time the spectrophotometer runs off scale. In many instances the new method required no attenuation at all.