Abstract
We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of hetero‐Diels–Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene‐modified model protein. Quantitative conjugation occurs over the course of a few hours, at ambient temperature and neutral pH, and in the absence of any catalyst. Our technology platform affords thermoresponsive bioconjugates, whose aggregation is solely controlled by the polymer chains.
Highlights
We introduce the bioconjugation of polymers synthesized by reversible addition-fragmentation transfer (RAFT) polymerization, bearing no specific functional end group, by means of hetero-Diels–Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene-modified model protein
Protein-polymer conjugation was performed by simple incubation of dienefunctionalized BSA (dBSA) with the RAFT polymers in aqueous buffers, in the absence of catalyst and at room temperature
We observed that the conjugation of the first two polymer chains to dBSA occurred within just 1–2 h, with the fully conjugated protein obtained after 6 h
Summary
We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of hetero-Diels–Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene-modified model protein. Barner-Kowollik Centre for Materials Science, Queensland University of Technology (QUT), 2 George Street Brisbane, QLD 4000 (Australia) Barner-Kowollik School of Chemistry and Physics, Queensland University of Technology (QUT) 2 George Street, Brisbane, QLD 4000 (Australia) E-mail: christopher.barnerkowollik@qut.edu.au First protein-polymer conjugates obtained through the RAFTHDA pathway (Scheme 1).
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