Herpesvirus (HSV-1, EBV and CMV) infections in atherosclerotic compared with non-atherosclerotic aortic tissue.

Abstract

The viral nucleic acid of herpes simplex virus type 1 (HSV-1), Epstein-Barr virus (EBV) and cytomegalovirus (CMV) was studied by polymerase chain reaction (PCR), Southern blotting and in situ hybridization (ISH) in aortic tissues from 33 autopsies. In 23 cases involving persons who ranged from 23 weeks to 75 years of age at the time of death, the tissue was histologically non-atherosclerotic. Of these 23, aortic tissues tested positive for HSV-1 in 13%, for EBV in 13% and for CMV in 4%. In the other 10 cases involving persons who were 53-75 years old at death, atherosclerotic aortic tissue tested positive for HSV-1 in 80%, for EBV in 80% and for CMV in 40%. Neither double nor triple infections occurred in the non-atherosclerotic group, whereas six of 10 were positive for two viruses, and two of 10 were positive for three viruses in the atherosclerotic group. By in situ hybridization, the viruses were localized in cells morphologically consistent with endothelial cells and smooth muscle cells. We detected HSV-1, EBV and CMV DNA in cells in the upper portion of the non-atherosclerotic aortic wall, whereas viral DNA was detected more extensively in atherosclerotic lesions than in non-atherosclerotic tissue. We also are the first to show the existence of EBV DNA in the human aortic wall. In conclusion, we suggest that the high incidence and kinds of herpesviruses are related to the high incidence of atherosclerosis.