Abstract

Elution curves for aqueous solutions of NaCl and β-lactoglobulin were measured for columns packed with porous polyacrylamide gel or non-porous glass particles. The elution curves were mathematically represented by series of Hermite polynomials in terms of the moments of the chromatographic pulses. Fitting the representation to skewed output pulses provides a criterion for trimming tails on elution curves.

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