Abstract
Heritable quantitative and qualitative changes can be observed in the nuclear DNA of doubled-haploid (D.H.) plants obtained by pollen culture of Nicotiana sylvestris . D.H. plants resulting from consecutive androgenic cycles contain on the average increasing amounts of total DNA and increasing proportions of highly repeated sequences. All the results suggest that both A-T rich and G-C rich regions are amplified in the D.H. genome. Some hypotheses on the origin of the changes are discussed.
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