Abstract
Methods for separating the hereditary endosymbiotic association Paramecium bursaria into its two components, paramecium and chlorella and for the independent culture of each are described. Chlorella-less paramecia can be reinfected, and combinations of algae and paramecia of various strains have been established and analyzed. The results of such “recombination” experiments demonstrate differences among the chlorellae from diverse strains of P. bursaria and indicate as well that the host paramecia of diverse strains can be distinguished. These facts suggest that P. bursaria will prove an excellent material for general problems of cell heredity and endosymbiosis.
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