Abstract
BackgroundHemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously. DCs are a unique group of white blood cells that initiate primary immune responses by capturing, processing, and presenting antigens to T cells.ResultsIn the present study, we investigated the effect of HemoHIM on the functional and phenotypic maturation of murine bone marrow-derived dendritic cells (BMDCs) both in vitro and in vivo. The expression of co-stimulatory molecules (CD40, CD80, CD86, MHC I, and MHC II) and the production of cytokines (IL-1β, IL-6, IL-12p70, and TNF-α) were increased by HemoHIM in BMDCs. Furthermore, the antigen-uptake ability of BMDCs was decreased by HemoHIM, and the antigen-presenting ability of HemoHIM-treated mature BMDCs increased TLR4-dependent CD4+ and CD8+ T cell responses.ConclusionsOur findings demonstrated that HemoHIM induces TLR4-mediated BMDCs functional and phenotypic maturation through in vivo and in vitro. And our study showed the antigen-presenting ability that HemoHIM-treated mature BMDCs increase CD4+ and CD8+ T cell responses by in vitro. These results suggest that HemoHIM has the potential to mediate DC immune responses.
Highlights
HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously
HemoHIM is not cytotoxic to bone marrow-derived dendritic cell (BMDC) We investigated whether HemoHIM at different concentrations induces apoptosis in BMDCs
The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. OVA257-264-specific T cells co-cultured with untreated BMDCs. +, p < 0.05, ++, p < 0.01, and +++, p < 0.001 vs. OVA257-264-specific T cells co-cultured with OVA257-264-pulsed BMDCs
Summary
HemoHIM, which is an herbal preparation of three edible herbs (Angelicam gigas Nakai, Cnidium offinale Makino, and Peaonia japonica Miyabe), is known to have various biological and immunological activities, but the modulatory effects of this preparation on dendritic cells (DCs)-mediated immune responses have not been examined previously. DCs are the most potent antigen presenting cells (APCs) [1], originate from the bone marrow, and play a pivotal role in the induction of adaptive immunity as initiators of T cell responses against pathogens and tumors [2,3,4,5]. Mature DCs have lower antigen uptake abilities than immature DCs but express higher levels of co-stimulatory molecules and major histocompatibility complex class (MHC) I and II on their surfaces [1, 8]. These cells play key roles in the antigen-specific T cell responses that are required to initiate adaptive immune responses [2, 3, 9].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
