Abstract
IntroductionOne of the most serious complications of diabetes is the formation of Diabetic Foot Syndrome. Herbal extracts that combine high antioxidant and antimicrobial properties can be used to treat the resulting neuropathy.The aim of this study was to determine antimicrobial and antioxidant activities of crude extracts isolated from plants growing in Kazakhstan, which could be used to develop products for treatment of Diabetic Foot Syndrome.MethodDifferent solvents, including dichloromethane and ethanol, were used to prepare plant extracts. The crude extracts from the plants were tested for antimicrobial activity using a modified version of the CLSI/NCCLS methods. All organisms were obtained from American Type Culture Collection. These included the fungi Candida glabrata ATTC 90030, the bacteria Staphylococcus aureus ATCC 29213, and Methicillin-resistant S. aureus ATCC 43300. The 2,2-diphеnyl-1-picrylhydrazyl (DPPH) assay, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay were used to analyzed the antioxidant capacity.ResultsThe results clearly indicate that antibacterial and antifungal activities vary with plant species. Dichloromethane extracts produced favorable results in all assays. Epilobium hirsutum, Rhodiola quadrifida, Rumex confertus showed antifungal activity against Candida glabrata in all extracts where IC50 less than 3 μg/ml. Rumex confertus, Glycyrrhiza Uralensis and Vexibia alopecuroides showed anti-fungal activity against Staphylococcus aureus (IC50 =10.80 μg/ml), (IC50 =11.10 μg/ml), (IC50 =3.05 μg/ml) and Methicillin-resistant S. aureus (IC50 =16.20 μg/ml), (IC50 =11.00 μg/ml), (IC50 =2.90 μg/ml) respectively. In spite of this, Vexibia alopecuroides extract showed no antioxidant activity. The other extracts showed a dose dependent ABTS scavenging activity. IC50 values were for the following: 6.6 μg/ml Epilobium hirsutum; 4.5 μg/ml Rumex confertus; 3.8 μg/ml Rhodiola quadrifida, 5.7 μg/ml Glycyrrhiza Uralensis. Extracts of Epilobium hirsutum and Rumex confertus had high antioxidant activity greater than 85% inhibition of DPPH (P ≤ 0.05).ConclusionThe demonstrated antimicrobial and antioxidant activities showed evidence supporting the use of herbal extracts to treat Diabetic Foot Syndrome.
Highlights
One of the most serious complications of diabetes is the formation of Diabetic Foot Syndrome
This journal is published by the University Library System of the University of Pittsburgh as part of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press
Introduction: One of the most serious complications of diabetes is the formation of Diabetic Foot Syndrome
Summary
One of the most serious complications of diabetes is the formation of Diabetic Foot Syndrome. University, Almaty, Kazakhstan; 2Institute of Plant Biology and Biotechnology; 3School of Pharmacy, University of Missisippi, Oxford, Mississippi Herbal extracts that combine high antioxidant and antimicrobial properties can be used to treat the resulting neuropathy. The aim of this study was to determine antimicrobial and antioxidant activities of crude extracts isolated from plants growing in Kazakhstan, which could be used to develop products for treatment of Diabetic Foot Syndrome.
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