Herbal Antagonism of a Superbug – A Novel Approach

Abstract

Background: This study was conducted to herbally antagonize the growth of a multi drug resistant Pseudomonas sp using ethanolic carrot rough fiber extractive. Materials and methods: The isolated strain of Pseudomonas sp was subjected to treatment with 5 different antibiotics-Chloramphenicol, penicillin, streptomycin, amoxycillin clavulanate along with monocef up to a concentration of 500µg/ml. The isolated organism was also treated with a mutagen Ethyl methane sulphonate. The antimicrobial property of the ethanolic carrot rough fiber extractive was tested by well diffusion method. MIC study of the ethanolic carrot rough fiber extractive was also performed. Result: The isolated strain was found to be resistant against 5 different antibiotics-Chloramphenicol, penicillin, streptomycin, amoxycillin clavulanate along with monocef up to a concentration of 500µg/ml. This organism was also found to grow at 44°C without any pigment production. This Pseudomonas sp also possess the ability of biofilm formation and antagonize the growth of other microbial population. There is reduced growth of this superbug under the influence of Ethyl methane sulphonate. It also became susceptible to the antibiotics monocef, streptomycin and higher concentration of amoxicillin. So the isolated strain is very much prone to induced mutation. The growth of this multi drug resistant strain of Pseudomonas sp has been antagonized by the ethanolic carrot fibre extractive. MIC study of the ethanolic carrot rough fibre extractive was performed which revealed that the MIC lies between 125µl-150µl i.e., 216.6-260mg/ml concentration of the extract. Conclusion: The ethanolic carrot rough fibre extractive has been found to antagonize the growth of the multidrug resistant Pseudomonas sp.