Heptapeptide Isolated from Isochrysis zhanjiangensis Exhibited Anti-Photoaging Potential via MAPK/AP-1/MMP Pathway and Anti-Apoptosis in UVB-Irradiated HaCaT Cells.

Zhaowan Zheng,Yuan-Lin He,Zhong-Ji Qian,Yanfei Tang,Zhenbang Xiao,Chunxia Zhou,Hui Luo,Lefan Li,Pengzhi Hong

doi:10.3390/md19110626

Abstract

Marine microalgae can be used as sustainable protein sources in many fields with positive effects on human and animal health. DAPTMGY is a heptapeptide isolated from Isochrysis zhanjiangensis which is a microalga. In this study, we evaluated its anti-photoaging properties and mechanism of action in human immortalized keratinocytes cells (HaCaT). The results showed that DAPTMGY scavenged reactive oxygen species (ROS) and increase the level of endogenous antioxidants. In addition, through the exploration of its mechanism, it was determined that DAPIMGY exerted anti-photoaging effects. Specifically, the heptapeptide inhibits UVB-induced apoptosis through down-regulation of p53, caspase-8, caspase-3 and Bax and up-regulation of Bcl-2. Thus, DAPTMGY, isolated from I. zhanjiangensis, exhibits protective effects against UVB-induced damage.

Highlights

Skin aging is an irreversible natural physiological process related to free-radical formation in the skin [1]
The peptide Ala–Asp–Ala–Phe with the highest antioxidant activity was identified for the first time from walnut protein hydrolysate [27]
The presence of the sulfur-containing amino acid Met strengthens the antioxidant properties of peptides [30]

Summary

Introduction

Skin aging is an irreversible natural physiological process related to free-radical formation in the skin [1]. Under the current conditions of the Earth’s rotation, 280–3000 nm solar radiation reaches the Earth’s surface, among which the 280–1600 nm wavelengths can be divided into ultraviolet (UV), visible light (VIS), and near infrared (IR) [2]. Of the UV radiation that reaches the Earth’s surface, UVB (280–320 nm) is directly associated with photodamage and photoaging, as UVA (320–400 nm) confers approximately 1000-fold less photic damage than UVB [4]. ROS are very reactive free-radical molecules involved in cellular damage and molecular signaling, and they play a crucial role in skin aging [4]. UVB-induced skin injury can lead to a large number of ROS-mediated signal transduction abnormalities, cell redox homeostasis imbalance, and excessive apoptosis. The use of various antioxidants to control UVB-induced damage is considered an important strategy

Methods

Results

Conclusion