Hepatotoxic effect evaluation of probiotic medication of candidates for elemental status correction of animals based on research of biochemical blood indicators

Abstract

The paper presents the data of possible application of probiotic medications, based on the bacteria of genus Bacillus as micronutrient medication for correction of mineral deficiency conditions that have the ability to accumulate metals, in order to deliver zinc-ions in the body of laboratory animals. The choice of the research objects of probiotic medications on the basis of bacteria Bacillus: Vetom 1.1 (the strain of B. subtilis 10641) and Vetom 3(the strain of B. amyloliquefaciens 10642) is conditioned by the high depositing characteristics given by the microorganisms of essential elements, in particular zinc, from the different inorganic compounds. The evaluation of accumulation of bioavailable zinc forms was carried out on the laboratory rats as models. The inactivated biomass of bacteria, after long periodical cultivation in a liquid nutrient medium with zinc sulfate, was orally injected in their bodies. One of the main criteria for the development of medication candidates besides the evaluation of delivery effectiveness is the absence of pathological effects on the body of laboratory animals. To implement this task, we evaluated the hepatotoxic effect of applied medications on the basis of research of alanine transaminase, aspartate transaminase and alkaline phosphatase level in a blood serum throughout the experiment. The obtained data indicates the absence of pathological effects on hepatocytes, as the concentration of researched biochemical indicators was within the reference values throughout the experiment.