Hepatoprotective properties for Salvia cryptantha extract on carbon tetrachloride-induced liver injury.

Abstract

The present study was designed to determine the possible hepatoprotective effects of Salvia cryptantha (black weed) plantextract againstcarbon tetrachloride (CCl4)-inducedhepatic injury inrats. Animals were grouped as follows: control group (Group I), CCl4 group (Group II), olive oil group (Group III), CCl4 +S. cryphantha200 mg/kg group (Group IV), and CCl4 +S. cryptantha400mg/kg group (Group V). Rats were injectedintraperitoneallywithCCl4 dilutedinolive oil(50% v/v) at adoseof1ml/kgbody weight. Bax and Caspase3were determined byimmunohistochemical staining, while apoptotic indexwas evaluated using TUNEL assay. Total mRNA was isolated from liver tissues, and the levels of BCL2, Caspase3, SOD, CAT, and glutathione peroxidase (GPx) were determined by using PCR, while MDA level were determined using a colorimetric assay. The antioxidant and anti-apoptotic gene transcripts were decreased in all of the control and treatment groups, while Caspase3 levels were not statistically different. The S. cryptantha plant extract treatment was also found to improve SOD, GPx, and catalase levels, while reducing the serum levels of MDA. The extract of S. cryptanthasupplementationhad a protective effect against CCl4-induced liver damage. S. cryptanthaextract as a supplement may be useful as a hepato-protective agent to combat the toxic effects caused by CCl4and other chemicals.