Abstract
Carbon tetrachloride (CCl4) is haloalkane that possesses a hepatotoxic effect. Material and Methods: The impact of anthocyanin fractions obtained from Hibiscus rosasinensis petal on carbon tetrachloride (CCl4) induced acute liver damage in wistar rats was studied using a combination of alanine transferase (ALT) activity value and liver: body weight gain ratio as indices. Results: CCl4 treatment significantly increased both ALT value and the liver: body weight gain ratio at the 1% probability level when compared with the control values. Conclusion: Pre-treatment with the anthocyanin fractions reduced the levels of these markers and hence, the degree of liver damage, though with varying potentials. The lead precipitated, non slimy red fraction possessed the greatest protective property on the rat liver when compared with the other anthocyanin fractions so tested. Keywords: Anthocyanin, carbon tetrachloride, alanine transferase, Hibiscus rosasinensis, liver damage.Sudan Journal of Medical Sciences Vol. 3 (1) 2008: pp. 33-36
Highlights
This study reports the protective capacities of partially purified extracts of H. rosasinensis petal anthocyanin against carbon tetrachloride-induced liver damage
alanine transferase (ALT) activity values are expressed as mean±SD of seven rats per group. *Significantly different from the control value (P
Measurement of plasma ALT activity value has been used as an index of acute liver damage induced by CCl4 exposure
Summary
Animals: Forty-nine (49) adult albino rats (Wistar strain) with an initial mean weight of 154±16g (135-170g) were used for the study. The rats were obtained from the Animal Unit, Faculty of Pharmacy, University of Benin, Benin City, Edo State, Nigeria. Collection of Hibiscus Flowers: Hibiscus flowers (Hibiscus rosasinensis) were collected from the environment in University of Benin, Ugbowo, Benin City. Extraction and Purification of Anthocyanins: Anthocyanins were extracted from flower petals and purified as previously described[5]. The extracts obtained from the stepwise purification techniques were respectively labelled: AN1, AN2, AN3, AN4 and AN5. AN1 is the crude anthocyanin extract and AN2, the lead precipitated extract, while AN3 is the 12 x 3.5cm sephadex G-50 column fraction. AN4 and AN5 are 50 x 10cm sephadex G–50 column fractions 1 and 2, respectively. AN2 was obtained from crude, while the column fractions were obtained from AN2
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