Hepatoprotective Potential of Endophytic Aspergillus Niger Strain A6 Fractions Isolated from Phyllanthus Amarus Schum and Thonn

Abstract

Aim To isolate characterize and screen for the in vitro antioxidant and hepatoprotective effects of P1EA and P1nB extracts from the endophytic fungi Aspergillus niger strain A6 PALF-1 isolated from leaves of Phyllanthus amarus in paracetamol induced hepatotoxicity.Materials and methods PALF-1 was isolated from Phyllanthus amarus leaves by standard procedures. Chloroform P1C ethyl acetate P1EA and n-butanol P1nB extracts were drawn up. P1C P1EA and P1nB were screened for in vitro antioxidant activity against 2 2-diphenyl-1-picrylhydrazyl DPPH hydroxyl radical followed by reducing power assay. P1EA 50 and 100 mgkg b. w. and P1nB 50 and 100 mgkg b. w. were screened for hepatoprotective activity against paracetamol model in rats. High performance thin layer chromatography HPTLC profile of P1EA and P1nB was also studied. Characterization of PALF-1 was done by polymerase chain reaction PCR sequential analysis.Results P1EA and P1nB found to scavenge DPPH hydroxyl radicals and also showed reductive ability. In animal studies administration of paracetamol 2 gmkg b. w. significantly increased the levels of liver biochemical marker enzymes in comparison to normal group. A marked decrease in the levels of superoxide dismutase SOD and catalase CAT was also observed. Administration of P1EA and P1nB 50 amp100 mg kg significantly reduced the actions of paracetamol by lowering the levels of serum biochemical parameters at various significance levels plt0.001 plt0.01 increasing the levels of SOD and CAT plt0.001 and decreased lipid peroxidation LPO. rDNA PCR sequencing analysis phylogenetic and molecular studies of PALF-1 confirmed the endophytic fungus as Aspergillus niger strain A6. HPTLC finger printing of P1EA and PnB showed the presence of polyvalent compounds.Conclusion P1EA and P1nB fractions of Aspergillus niger strain A6 showed significant antioxidant and hepatoprotective activity in paracetamol induced hepatotoxicity. This may be attributed to the antioxidant principles present in P1EA and P1nB which need to be explored.