Hepatoprotective liposomal glycyrrhizin in alcoholic liver injury

Abstract

IntroductionLicorice root (Glycyrrhiza glabra L.) extract and its main active component glycyrrhizin, as well as derivates are well known hepatoprotective and gastroprotective agents. Liposomal applications of these compounds may prevent low bioavailability and side-effects, e.g. pseudoaldosteronism and has further benefits, because the nanoparticles with glycyrrhizin and glycyrrhetinic acid have enhanced uptake into the liver. Our aim was to study the hepatoprotective effect of liposomal glycyrrhizin treatment during long-term alcohol consumption in rats. MethodsHarlan-Wistar (175–200g) rats were randomly allocated into three groups: control, alcoholic, ethanol and liposomal glycyrrhizin-treated. The redox status of the liver was studied with the measurement of free sulfhydryl-group concentration and hydrogen-donor ability. Transmethylation capacity was measured in dimedone adduct form. Histological examination was carried out as well. ResultsData showed mild elevation in the hydrogen-donor ability and slightly lowered free sulfhydryl level, although transmethylation capacity was significantly lowered (p<0,05) in the alcoholic group. Liposomal glycyrrhizin caused no marked histological changes. Effect of the treatment on the redox homeostasis and transmethylation capacity was beneficial. ConclusionsLiposomal glycyrrhizin treatment moderated the alcohol-related negative changes, therefore glycyrrhizin and/or its derivatives may have further impact in the treatment of alcoholic liver diseases.