Hepatoprotective Efficacy of Sharbat-e-Deenar Against Carbon Tetrachloride−Induced Liver Damage

Abstract

This study was undertaken to evaluate the antioxidant and hepatoprotective efficacy of Sharbat-e-Deenar (SD) against carbon tetrachloride (CCl(4))-induced hepatic damage in a rat model. The antioxidant activity of SD was estimated by DPPH assay, H(2)O(2) assay, and total phenolic contents. SD therapy at doses of 1, 2, and 4 mL/kg, orally, was administered after CCl(4) intoxication (1.5 mL/kg, intraperitoneally for 48 hours) in experimental animals. Hexobarbitone sleep time and bromosulfophthalein retention time also were determined against CCl(4)-induced liver damage. Exposure to CCl(4) in experimental animals showed biochemical and histopathological deterioration in the liver. Treatment with SD showed a marked protection on biochemical parameters, that is, alanine transaminase, aspartate transaminase, albumin, and urea. SD therapy exhibited a protective effect by restoring the level of lipid peroxidation-reduced glutathione, adenosine triphosphate, and glucose-6-phosphatase (G-6-Pase). Treatment with SD significantly recovered the level of hexobarbitone sleep time and bromosulfophthalein retention time. DPPH and H(2)O(2) assays showed antioxidant properties in a dose-dependent manner. Histopathological observations of liver sections showed recovery in liver architecture after SD treatment. These results indicate that SD exerts a protective effect on CCl(4)-induced hepatotoxicity, which may be due to its antioxidant properties.