Abstract
Objective: The aim of this current study is to investigate the hepatoprotective efficacy of N-Miracle (a polyherbal formulation) against ethanol-induced toxicity in male albino rats.
 Methods: Male Wistar albino rats weighing 150-200 g were used for the study. A total of 30 male albino rats were selected, divided into five groups. Ethanol-induced liver damage was done on Group III, IV, and V. Group I and Group II served as a normal and drug (N-Miracle) control. After the treatment period, the rats were anaesthetized by light ether anesthesia in a lethal chamber. Hepatic biomarkers, antioxidant enzymes, histopathological examination are carried out to document the hepatoprotective effect of N-Miracle (Polyherbal formulation).
 Results: The results of the present study demonstrated a significant (p<0.05) increase in the levels of Aspartate Aminotransaminase (AST), Alanine Aminotransaminase (ALT) and Alkaline Phosphatase (ALP) in ethanol-induced rats as compared to normal and drug control Groups. The level of total protein and albumin were significantly (p<0.05) decreased in ethanol-treated rats. The toxic impact of ethanol was found to be restored in rats treated with N-Miracle (Polyherbal formulation). The present study also exhibited the enzymatic antioxidant efficacy of N-Miracle (Polyherbal formulation) against ethanol-induced toxicity in rats by increasing the antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase and decreasing the activity of Glutathione-S-transferase in the liver. The findings are also correlated with histopathological examination of N-Miracle treated group, which shows hepatic regeneration and decrease in degradation of hepatocytes.
 Conclusion: This study could provide a possible explanation to hepatotoxicity resulting from exposure to ethanol. The findings of the present study revealed the ameliorative effect of N-Miracle (Polyherbal formulation) against ethanol-induced hepatotoxicity by improving the liver function, increasing the levels of antioxidant enzymes and restoring the morphological features of the liver.
Highlights
The liver is the chief vital organ and the site for necessary biochemical reactions in the human system
Acetaldehyde metabolic product of alcohol is more lethal than alcohol and it is connected with a more number of the metabolic disorders in liver disease triggered by alcohol
We found that there was significant (p0.05) increase in total protein in N-Miracle (Polyherbal formulation) (Group IV) compared to ethanol treated rats (Group III)
Summary
The liver is the chief vital organ and the site for necessary biochemical reactions in the human system. Hepatotoxicity may result from direct toxicity of the primary compound and/or from a reactive metabolite or from an immunologically-mediated response affecting hepatocytes, biliary epithelial cells and/or liver vasculature[1] Ethanol toxicity was discovered to be a major cause of liver disorder for centuries. It is a growing medical and public health issue challenged by the adult and adolescent populations. Alcohol ingestions have been found to cause the build-up of reactive oxygen species, which is the basis of lipid peroxidation of cellular membranes and proteins as well as DNA oxidation, resulting in liver cell necrosis [4]. Herbal remedies are proven to play a vital role in the treatment of liver diseases [5]
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