Hepatoprotective Effect of Inularacemosa on Hepatic Ischemia/reperfusion Induced Injury in Rats

Abstract

Introduction: Hepatic Ischemia-Reperfusion (I/R) injury contributes to organ injury and dysfunction after hepatic surgery and transplantation. I/R induce Kupffer cell activation, leading to the release of pro-inflammatory cytokines that promote injury, increase adhesion molecule expression, and facilitate polymorphonuclear neutrophil injury. Inularacemosa contains high concentrations of the flavonol glycosides, which has been shown effect on cardiac function and oxidative stress against isoproterenol – induced myocardial infarction. Historically, the roots were reputed to have Anti-inflammatory and Analgesic effects. The hepatoprotective activity of the drug against hepatic Ischemia/Reperfusion injury has not been reported yet. Objective: To study the hepatoprotective effect of hydroalcholic extract of Inularacemosa at 200 and 400 mg/kg against hepatic ischemia/reperfusion injury. Methodology: 24 male wistar rats were divided in to four groups. The normal control group, model control group and extract treated group at a dose of 200 and 400 mg/kg were orally fed with distilled water as vehicle for 21 days followed by ischemia/reperfusion on twenty second day. Blood and liver samples were obtained from all the animals on 22nd day for biochemical analysis of AST, ALT, ALP and LDH and histopathological studies were also performed. Results: The results showed that the ischemia/reperfusion injury causes significant increase in the levels of AST, ALT, ALP and LDH in model control group indicating the cell damage and tissue injury whereas supplementation with hydroalcholic extract of Inularacemosa significantly reduced the elevated levels of above parameters. Histopathological analysis showed high degree of congestion and mild necrosis in model control group which was reduced to minimum levels in drug treated groups. Inularacemosa increased the free radicals scavenging activity in the early period of hepatic IR injury in rats. Conclusion: Reduced level of liver enzymes and histopathological studies evident that Inularacemosa possesses beneficial effects on the hepatocytes in hepatic I/R injury.