Abstract
Tuberculosis (TB) is one of the leading causes of death in developing countries. One of the problems in controlling TB disease is that most anti-tuberculosis drugs are hepatotoxic. Citrus sinensis peel extract is the rich source of secondary metabolites with high potential effectiveness as an antioxidant. In the present study, we evaluate the hepatoprotective effect of Citrus sinensis peel ethanolic extract (CSPEE) on isoniazid and rifampicin-induced liver injury in Wistar rats. Twenty five adult male Wistar rats were divided into 5 groups of 5 each : control, (INH+RIF) (50 mg/kg bw once a day for 14 days), (INH+RIF) + various dose of CSPEE (300, 450, 600 mg/kg bw). CSPEE was given orally once a day for 14 days followed by administration of INH + RIF suspension. The measurement of serum ALT and AST were carried out on the 15th day. Histopathologic examination of the liver was also performed. The Serum ALT and AST of the rats that induced with INH + RIF were increased significantly (P<0.001) compare to those of control groups, and the histopathologic slides showed steatosis, vacuolation and necrosis of hepatic cells. The serum ALT and AST in groups treated with CSPEE were not significantly different (p>0.05) with those of control groups. The serum ALT and AST and histopathological examination of the liver of the group that administered 600 mg/kg CSPEE were closest to normal rats. Citrus sinensis peel extract exhibits hepatoprotective effect on liver injury induced with INH + RIF in Wistar rats.
Highlights
Drug-induced hepatotoxicity is the most common cause of acute liver failure in the United States (Ostapowicz et al 2002)
Citrus sinensis peel extract exhibits hepatoprotective effect on liver injury induced with INH + RIF in Wistar rats
Several studies on the benefits of sweet orange peel extract (Citrus sinensis) have been done such as: evaluation of anti-inflammatory, antibacterial and antioxidant properties (Omodamiro & Umekwe, 2013; Mehmood et al, 2015; Shetty et al, 2015; Hassan et al, 2017); antidiabetic and anticholesterol activities in mice (Muhtadi et al, 2015; Ernawita et al, 2017); protective effect on the onset of oxidative stress and alcohol-induced ulcus gastricus in mice (Selmi et al, 2017); improvement of liver functions of animal trials induced by cigarette smoke. (Setyawati & Anggraeni, 2018); and the effectiveness of Citrus sinensis peel methanolic extract as a hepatoprotector against liver damage and oxidative stress induced by CCl4 in Wistar rats (Ikeokwu, 2014)
Summary
Drug-induced hepatotoxicity is the most common cause of acute liver failure in the United States (Ostapowicz et al 2002). Several studies on the benefits of sweet orange peel extract (Citrus sinensis) have been done such as: evaluation of anti-inflammatory, antibacterial and antioxidant properties (Omodamiro & Umekwe, 2013; Mehmood et al, 2015; Shetty et al, 2015; Hassan et al, 2017); antidiabetic and anticholesterol activities in mice (Muhtadi et al, 2015; Ernawita et al, 2017); protective effect on the onset of oxidative stress and alcohol-induced ulcus gastricus in mice (Selmi et al, 2017); improvement of liver functions of animal trials induced by cigarette smoke. This study aimed to examine the protective effect of citrus sinensis (L) peel ethanolic extract (CSPEE) against liver injury induced by INH and RIF in male white rats (Rattus norvegicus) Wistar strain
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