Hepatoprotective Effect of Biofield Energy Treatment On Tert-Butyl Hydro Peroxide Induced Liver Injury in Hepatocellular Carcinoma Cell Line (HepG2)

Mahendra Kumar Trivedi ,Snehasis Jana

doi:10.29011/2576-9588.100029

Abstract

Author(s): Trivedi, Mahendra Kumar; Jana, Snehasis | Abstract: The present study was performed to investigate the Hepatoprotective potential of the Biofield Energy (The Trivedi Effect®) Treated test item, Dulbecco’s Modified Eagle Medium (DMEM) in HepG2 cells. The test item was distributed into two parts. One part received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and was labeled as the Biofield Energy Treated DMEM group and the other part referred as the untreated DMEM group, where no Biofield Treatment was provided. Results showed that more than 97% cell viability of the test items were observed by MTT assay, which indicated a safe and nontoxic nature of the test items. The Biofield Treated DMEM showed significant (p≤0.001) protection of cells by 10% against oxidative stress induced by t-BHP, while the untreated DMEM group showed 0.4% protection. The level of interleukin-8 (IL-8) was significantly (p≤0.01) reduced by 31.57% in Biofield Treated DMEM than untreated DMEM. The level of ALT enzyme activity was significantly (p≤0.001) reduced by 66% in Biofield Treated DMEM compared to untreated DMEM. Cholesterol level was significantly (p≤0.001) reduced by 46.23% in Biofield Treated DMEM than untreated DMEM. Besides, Biofield Treated DMEM group showed 51.18% increased the level of albumin compared to untreated DMEM group. Overall, results demonstrated that Biofield Treatment significantly protect the liver hepatocytes against oxidative stress. Therefore, Consciousness Energy Healing (The Trivedi Effect®) Treatment might be useful as a hepatoprotectant against different types of liver injuries like cirrhosis, alcohol abuse, hemochromatosis, Wilson’s disease, Gilbert’s disease, cholangiocarcinoma, steatosis, Budd-Chiari syndrome, etc.

Highlights

The liver is a vital organ actively involved in most of the metabolic functions
The Biofield Energy Treated test item (DMEM) showed a significant (p≤0.001) protection of cells by 10% from the oxidative damage induced by tert-Butyl hydroperoxide (t-BHP), while the untreated Dulbecco’s Modified Eagle Medium (DMEM) group showed 0.4% protection
IL-8 level was significantly (p≤0.01) reduced by 31.57% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group

Summary

Introduction

The liver is a vital organ actively involved in most of the metabolic functions. Hepatic damage is associated with distortion of these metabolic functions [1]. Human hepatoma cell lines (HepG2) have been routinely used as an alternative model to human hepatocytes in vitro for the evaluation of Hepatoprotective activity [7,8]. This cell line has many advantages viz. Available and cryopreserved in huge quantity, and even the drug metabolizing capability do Citation: Trivedi MK, Jana S (2018) Hepatoprotective Effect of Biofield Energy Treatment On Tert-Butyl Hydro Peroxide Induced Liver Injury in Hepatocellular Carcinoma Cell Line (HepG2). The authors focused to develop an alternative treatment to investigate the potential of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the test item, Dulbecco’s Modified Eagle Medium (DMEM) for protection of hepatocyte cells in Human Hepatoma Cells (HepG2). Biofield Therapy or Healing Modalities is one of treatment approach under the broad category of Complementary and Alternative Medicine (CAM) therapies to enhance physical, mental, and emotional human wellness

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Results

Conclusion