Abstract
To scientifically clarify the hepatoprotective constituents of Fructus Schizandrae chinensis, eleven batches samples of total dibenzocyclooctadiene lignans (TDL) from Schisandra chinensis were prepared by using the optimum extraction technique. Characteristic high-performance liquid chromatography (HPLC) chromatograms were obtained through HPLC analysis technology, and the hepatoprotective effects of the eleven batches of TDL were evaluated by MTT assay. Based on the chemical and biological activity results, the spectrum-effect relationship between the characteristic HPLC fingerprints and the hepatoprotective effect of TDL was established using Minitab 16.0 data analysis software. On the basis of the spectrum-effect relationship, thirteen compounds (1–13) were obtained from the TDL by chemical natural product chemical separation and purification technology, and their structures were identified on the basis of the spectral data and the literature. Based on these compounds, thirteen common peaks among the thirty-three chromatographic peaks in the above HPLC fingerprints were identified. Our findings showed that some components, including, schisandrin B (2), schisandrin A (3), and schisandrol B (7) had significant roles in promoting hepatoprotective activity. Preliminary verification of the spectrum-effect relationship of TDL from S. chinensis was carried out, and the results confirmed that the activity of a composite of these three key components in optimal ratios was better than that of any individual compound, which potentially confirmed the reliability of the spectrum-effect relationship and the synergistic effects of traditional Chinese medicine.
Highlights
Preliminary verification of the spectrum-effect relationship was carried out, and the results indicated that the activity of three key compounds in the optimal ratio was better than that of any individual compound
Many studies have proved that the spectrum-effect relationship should be an effective method to control the quality of traditional Chinese medicine (TCM) because it can connect chromatographic fingerprints with biological activity to explore the quality markers related to clinical efficacy [35]
The results showed that the activity of the three key compounds combined in the optimal ratio was better than that of any individual compound, which was consistent with the result of the spectrum-effect relationship
Summary
The dry fruit of Schisandra chinensis (Turcz.) Baill. Is a basic source plant of Fructus. Schisandrae chinensis and is officially listed in the Chinese Pharmacopoeia. As a wellknown traditional Chinese medicine (TCM), S. chinensis has five flavors, including sour, sweet, bitter, spicy, and salty. It is usually called Wuweizi [1]. S. chinensis is mainly distributed in Liaoning, Jilin, and Heilongjiang provinces and the North China region. A modern pharmacological study of dibenzocyclooctadiene lignans showed that they had many biological functions, such as liver protection, reduced enzyme levels, antibacterial, antitumour, antioxidant, antiviral effects, and immune enhancement [2,3,4,5,6]
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