Hepatoprotective activity of Tridecan-1-ol isolatedfrom Flaveria trinervia (Speng). C. Mohr

Abstract

Background- Flaveria trinervia (Asteraceae), a medicinal herb has been used in Indian traditionl medicine to cure jaundice. Reports on phytochemical and pharmacological investigations are very scarce. A phytoconstiuent Tridecan-1-ol and screened for hepatoprotective activity. -To evaluate hepatoprotective effects of Tridecan-1-ol against CCl4 induced hepatic damage on Wister albino rats. Methods- Tridecan-1-ol was isolated from petroleum extract of Flaveria trinervia using silica gel column chromatography and the structure was confirmed by IR, MASS,and 1H NMR spectroscopic studies. The parameters employed to confirm the hepatoprotectivity were the estimation of liver function serum markers such as total bilirubin, total protein, alanine transaminase, aspartate transaminase, alkaline phosphatase activities and the histological profile of the liver tissue. Results-The LD50 of petroleum ether extract and the constituent Tridecan -1-ol was found to be 500 and 150 mg/kg body weight respectively. Tridecan -1-ol showed significant hepatoprotective activity than the crude extract and it was compared with the standard hepatoprotective drug silymarin. The histological profile of the liver tissue ofTridecan-1-ol administered rats showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration as similar to the controls. Conclusion-Tridecan-1-ol afforded significant hepatoprotectivity against CCl4 induced liver damage and supported the traditional claim of Flaveria trinervia as a hepatoprotective herb.