Abstract
Introduction and Aim: Plants have become the current focus of research in treating the various diseases and ailments. Flacourtia jangomas (Lour.) Raeusch belongs to the familySalicaceae. Itis a small deciduous fruit tree having immense nutritional and medicinal significance. Different parts of the plant are pharmaceutically used forcuring various ailments. In this study, we investigated the hepatoprotective activity of Flacourtia jangomas (Lour.) Raeusch leaves and fruit methanolic extract on Paracetamol induced HepG2 cell line.
 
 Methods: The cytotoxic and hepatoprotective properties were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); lipid peroxidation (malondialdehyde (MDA) levels).
 
 Results:The increased cell viability of 140.43± 4.07% and 133.93±3.20%was observed in HepG2 cells treated with methanolic extract of F. jangomas leaf and fruit extract respectively at 10µg/ml concentration and then decreased along with the rise of F. jangomas leaf and fruit extract concentrations. The level of LDH, ALT, AST and MDA decreased after F. jangomas leaf and fruit treatment compared to negative control.
 
 Conclusion: This study suggests that the methanolic Extract of F. jangomas (Lour.) Raeusch leaves(FJL)and fruit (FJF) shows hepatoprotective activity in Paracetamol induced HepG2 cell line by the decrease in AST and ALT activities and LDH and MDA level. Hence, it could be considered as a therapeutic agent in curing liver-related diseases.
Highlights
Introduction and AimPlants have become the current focus of research in treating the various diseases and ailments
This study suggests that the methanolic Extract of F. jangomas (Lour.) Raeusch leaves (FJL) and fruit (FJF) shows hepatoprotective activity in Paracetamol induced HepG2 cell line by the decrease in AST and ALT activities and Lactate dehydrogenase (LDH) and MDA level
N-acetyl-para-benzo-quinone imine (NAPQI) can be detoxified by covalent binding with glutathione (GSH) in case of non-toxic consumption of paracetamol and when ingested at hepatotoxic doses the majority of the drug is metabolized by CYP2E1 pathway resulting in glutathione depletion which binds to macromolecules triggering oxidative stress, mitochondrial dysfunction, and resulting in hepatocellular death [2, 3]
Summary
Introduction and AimPlants have become the current focus of research in treating the various diseases and ailments. Flacourtia jangomas (Lour.) Raeusch belongs to the family Salicaceae. It is a small deciduous fruit tree having immense nutritional and medicinal significance. We investigated the hepatoprotective activity of Flacourtia jangomas (Lour.) Raeusch leaves and fruit methanolic extract on Paracetamol induced HepG2 cell line. Free radicals or reactive oxygen species (ROS) may cause oxidative stress leading to improper functioning of liver. NAPQI can be detoxified by covalent binding with glutathione (GSH) in case of non-toxic consumption of paracetamol and when ingested at hepatotoxic doses the majority of the drug is metabolized by CYP2E1 pathway resulting in glutathione depletion which binds to macromolecules triggering oxidative stress, mitochondrial dysfunction, and resulting in hepatocellular death [2, 3]
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