Abstract
Senggugu is a plant that has long been used to treat syphilis, typhoid, cancer, jaundice, and hypertension. The pharmacological activity of senggugu in Indonesia that have been reported include antifertility activity in leaves, mucolytic activity, anti-inflammatory and tracheospasmolytic, also antioxidant in its root bark. This study aims to determine the hepatoprotective effect of ethyl acetate extract fraction of senggugu’s root bark in rats induced by CCl 4 . The powder of senggugu’s root bark was extracted by terraced maceration method starts from n -hexane, ethyl acetate, to methanol, thus obtained ethyl acetate extract fraction of senggugu’s root bark (FEAKAS). The ethyl acetate extract fractions were then tested for hepatoproctective activity using doses of 25, 50, and 100 mg/Kg.BW on rats induced by CCl 4 . FEAKAS hepatoprotective activity was determined from the analysis of blood biochemical and oxidative stress parameters. The blood biochemical parameters included SGOT (serum glutamic oxaloacetic transaminase), SGPT (serum glutamic pyruvic transaminase), ALP (alkaline phosphatase), bilirubin, and total protein were measured with test kit. The oxidative stress parameters were measured from homogenates of liver tissue that were prepared by adding 500 mL of 50 nM Tris buffer (pH 7.4) containing 1 mM EDTA and 10 µg/mL leupeptin. The homogenates were centrifuged to obtain supernatants for measurement of oxidative stress parameters using spectrophotometer method, including MDA (malondialdehyde), GPx (glutathione peroxidase) and CAT (catalase). The results showed that the effect of FEAKAS against CCl 4 induction for preventing lipid peroxidation, from both blood chemical and oxidative stress parameters, are shown at a dose of 100 mg/Kg.BW that significantly different compared to CCl 4 control (ρ <0.05) on all blood chemical and oxidative stress parameters.
Highlights
Liver is one of the vital organs in the body that plays an essential role in regulating various physiological processes
We reported that the hepatoprotective activity of the EAFCS root bark in rats induced by carbon tetrachloride (CCl4)
The results indicated that induction with CCl4 at dose 1mL/Kg.BW caused hepatic damage of the rats and pretreatment with EAFCS at the dose of 100mg/Kg.BW protected hepatic damage
Summary
Liver is one of the vital organs in the body that plays an essential role in regulating various physiological processes. It plays a pivotal role in metabolism and distribution of nutrients as well as detoxification of toxic metabolites and xenobiotics (Chiang, 2014). It is involved the maintenance, performance and regulating homeostasis of the body. The liver is involved with almost all the biochemical pathways to growth, fight against the disease, nutrient supply, energy provision and reproduction (Ward and Daily, 1999).
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