Hepatoprotective activity of aerial parts of Erythrina crista-galli

Nahla Ayoub,Mohamed Ashour,Ashraf Abdel-Naim,Naglaa Mostafa

doi:10.21608/aps.2017.10357

Nahla Ayoub, Mohamed Ashour + Show 2 more

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https://doi.org/10.21608/aps.2017.10357

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Abstract

Hepatoprotective activity was measured for Erythrina crista-galli extract as well as fractions. Fractions II and III have shown a remarkable protective effect against CCl4-induced hepatocyte injury. This was evidenced by their ability to significantly ameliorate CCl4-induced elevation in ALT and AST levels. This is supported by the notion that pretreatment of hepatocytes with either Erythrina crista-galli extracts or fractions significantly alleviated CCl4- induced GSH and SOD depletion and replenished CCL4 reduction of TAC. Hepatoprotective activity mechanism is attributed at least in part, to the free radical scavenging and antioxidant activity of the phenolic compounds present in the extract proved by the phytochemical screening of the fractions.

Highlights

Phenolic compounds are commonly found in both edible and non-edible plants and they have been reported to have multiple biological effects
Since only a few reports are available in the current literature about the hepatoprotection activity of the leaves of Erythrina crista-galli, it was, found interesting to subject the extracts and fractions of the leaves as a continuation to our previous study where we isolated polyphenols with a phytoestrogen activity, here we aim to assess the hepatoprotective activity for the extract and fractions
In vitro pretreatment of HepG2 hepatocytes with the aqueous extract concentrations significantly replenished CCl4-induced Superoxide dismutase (SOD) reduction by 157% (2.5 folds), 69% and 4% respectively and replenished CCl4-induced GSH reduction by 120% (2.2 folds), 83% and 79% respectively .While aqueous methanol extract replenished CCl4-induced SOD reduction by 98%, 69% and 7.5% respectively and replenished CCl4-induced GSH reduction by 125%, 99% and 77% respectively

Summary

Introduction

Phenolic compounds are commonly found in both edible and non-edible plants and they have been reported to have multiple biological effects. Leguminosae which include genera that embrace phenolic-rich species is capable of synthesizing and accumulating the high percentage of phenolics flavonoids [1]. The genus Erythrina (Leguminosae) contains more than 110 species with a broad distribution [2]. These species have been widely used in indigenous traditional medicine [3] Erythrina has been used in folk medicine for treatment of insomnia malaria fever, venereal disease, asthma, and toothache. The alkaloid Erythroidine was used as a muscle relaxant. Haemoerythrina alkaloids were investigated for their anti-cancer activity [4]

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