Abstract
Objective: to evaluate the hepatoprotective efficacy of a complex compound of 5-hydroxy-6-methyluracil and succinic acid in experimental peritonitis. Materials and methods. Experiments were carried out on 48 male albino rats in which peritonitis was simulated via intraperitoneal administration of 7% fecal suspension in a dose of 0.6 ml per 100 g bodyweight. The rate of free radical oxidation processes, the activity of antioxidative protection, the degree of endogenous intoxication and cytolytic syndrome, and the effect of the test compound on these parameters were estimated in the experiment. Results. With the development of an abdominal inflammatory process, there were increases in rates of endogenous intoxication and free radical oxidation (FRO), a change in the activity of antioxidative protection enzymes, and a reduction in the levels of ceruloplasmin and sulfahydryl groups. The complex compound, that comprised 5-hydroxy-6-methyluracil and succinic acid used as monotherapy, reduced the degree of endogenous intoxication, FRO, and lipid peroxidation-antioxidative defense system imbalance. Conclusion. The experimental data suggest that the use of the complex compound containing succinic acid and 5-hydroxy-6-methy-luracil is pathogenetically warranted. Key words: peritonitis, lipid peroxidation, antioxidants, succinic acid, pyrim-idine derivatives.
Highlights
Experiments were carried out on 48 male albino rats in which peritonitis was simulated via intraperitoneal administration of 7% fecal suspension in a dose of 0.6 ml per 100 g bodyweight
The rate of free radical oxidation processes, the activity of antioxidative protection, the degree of endogenous intoxication and cytolytic syndrome, and the effect of the test compound on these parameters were estimated in the experiment
The complex compound, that comprised 5 hydroxy 6 methyluracil and succinic acid used as monotherapy, reduced the degree of endogenous intoxication, free radical oxidation (FRO), and lipid peroxidation antioxidative defense system imbalance
Summary
Работа выполнена на 48 крысах самцах массой 180—240 г. Всех животных содержали на стандартной диете вивария, эксперименты выполнены при температуре воздуха 20—21°С и нормальных показателях барометрического давления. В группе I экспериментальный перитонит воспроизводили у крыс путем внутрибрюшинного введения 7% каловой взвеси в дозе 0,6 мл/100 г массы. Для приготов ления гомогената печени навеску ткани отмывали охлажден ным физиологическим раствором, измельчали и заливали фо сфатным буфером (1/15М, рН 7,4) в соотношении 1:10, гомогенизировали при температуре 0 +4°С. Содержание SH групп определяли после образования меркаптида в результате взаи модействия n хлормеркурибензоната с SH соединениями с возрастанием оптической плотности раствора [11]. Для оценки интенсивности эндотоксикоза определя ли содержание олигопептидов (ОП) и веществ низкой и сред ней молекулярной массы (ВН и СММ) в плазме крови по мето дике М. Регистрировали спектральную характеристику водного раствора супернатанта при длинах волн от 238 до 306 нм. Математическую обработку выполняли на компьютере с при менением программного обеспечения Microsoft Excel
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