Hepatoprotective Activity and Molecular Docking Studies of Actinidia deliciosa Fruit Peel Extract Phytocompounds

Abstract

In the case of liver illnesses, herbal treatment offers a non-invasive, all-natural alternative to conventional medicine. Methanolic extract of the peel of Actinidia deliciosa was screened for its hepatoprotective in rodent models with antioxidant activity in-vitro H2O2 radical with hydroxyl radical scavenging assay was also used to assess the extract’s antioxidant potential. The gold standard was ascorbic acid. Various phytochemical constituents were identified, such as flavonoids, terpenoids, phenols, tannins, and alkaloids. In-vivo, hepatoprotective activity was performed by using paracetamol (3 g/kg)and ethanol (12 mL/kg) induced hepatotoxicity models. Total protein and albumin levels were raised, while SGPT, SGOT, cholesterol, and bilirubin were dramatically lowered. The extract effectively mopped up the harmful free radicals. However, a histological examination was also conducted in our investigation to determine the specifics of the damage caused by the hepatotoxic chemical and the plant’s potential to repair the hepatic structure. Several investigations have also shown that hepatoprotection is directly linked to antioxidant capacity. Molecular docking experiments investigated interactions with the active site of CYP450 2E1. By decreasing its expression, CYP2E1 prevents APAP bioactivation, decreasing NAPQI and protecting glutathione (GSH) levels. From the results, it is clear that to methanolic extract of A. deliciosa possesses hepatoprotective and antioxidant activities.