Hepatoprotective Activities of Black Soybean in High Cholesterol Diet‐induced Non‐alcoholic fatty liver disease

Abstract

Non‐alcoholic Fatty Liver Disease(NAFLD) is characterized by an increase in intra‐hepatocellular triglycerides, which is not due to alcohol. This study investigated the effect of black soybean supplementation on high cholesterol diet induced NAFLD. Mice were divided into five groups: normal control(NCD), high cholesterol control(HCD), and the high cholesterol diet supplemented with black soybean powder(1B‐HCD:1%, 2BHCD: 2%, 4B‐HCD:4%). Blood glucose, insulin, lipid profiles, adiponectin, and malondialdehyde(MDA) were determined. Blood glucose and insulin in HCD(163.2mg/dl, 3.07μIU/ml) were higher than in NCD(100.0mg/dl, 1.62μIU/ml), reducing in all experimental groups(129.2mg/dl, 1.76μIU/ml; 98.6mg/dl, 2.30μIU/ml; 113.7mg/dl, 2.29μIU/ml). Total cholesterol and triglyceride were only decreased in 4B‐HCD compared to HCD. Adiponectin was significantly decreased in HCD(6.68ng/ml), while increased in experimental groups(8.13ng/ml; 8.89ng/ml; 9.12ng/ml). MDA was dropped in all experimental groups(20.04μM; 33.89μM; 15.89μM), compared to HCD(35.96μM). The results indicate that supplementation of black soybean may improve lipid profiles and oxidative damage in NAFLD. This work was supported by SRC and BK 21.Grant Funding Source: Brain Korea 21